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How to Check Your Fertility: What Each Test Actually Indicates, for Women and Men

4 November 2026Lindi Collett (B.Dietetics)

"Fertility test" is a term that covers a lot of ground, and much of what's searched for online doesn't distinguish between the different things it can mean. A fertility test might refer to checking ovarian reserve, confirming ovulation, assessing sperm health, or reviewing a broader hormone panel - different questions, each answered by a different marker.

It's worth being direct about something upfront: no currently available fertility test, on its own, can tell you whether or when you'll conceive. Each one has a specific, narrower purpose. Results are best understood as one piece of information to discuss with a GP or fertility specialist - not a forecast.

This guide covers what each commonly tested marker actually indicates, for women and men.

AMH: what ovarian reserve testing shows, and doesn't show

Anti-Müllerian hormone (AMH) reflects the size of the remaining egg supply - your ovarian reserve. Levels tend to be lower when ovarian reserve is diminished, and are often elevated in polycystic ovary syndrome (PCOS) [1,2].

It's important to be precise about what a single AMH result does not do. On its own, it does not reliably predict whether or when you'll conceive naturally, and it shouldn't be used to counsel women - whether fertile or having difficulty conceiving - about their month-to-month chance of pregnancy [2,3,4].

AMH is also not a precise predictor of exactly when you'll reach menopause years in advance. That said, it isn't meaningless here either: very low or undetectable levels in a younger woman can signal an increased risk of early (premature) ovarian insufficiency, and AMH-based estimates of menopause timing become more accurate as menopause draws nearer [5,6].

Where AMH is genuinely useful is in planning fertility treatment: it helps a specialist anticipate how many eggs the ovaries are likely to produce in response to stimulation during IVF. Even then, it predicts the quantity of response, not the likelihood of a live birth - and a low result on its own is not a reason to be refused treatment. Outside that context, it's a single data point about ovarian reserve, best interpreted by a GP or fertility specialist alongside the full clinical picture [7].

Progesterone and ovulation: confirming a process, not predicting an outcome

A progesterone blood test is timed to roughly one week before your next expected period - not a fixed calendar day - and can provide presumptive evidence that ovulation is likely to have occurred in that cycle [8]. "Presumptive" is the key word: it's a strong indicator, not definitive proof, and a single measurement can't assess the quality of the luteal phase, because progesterone levels fluctuate considerably even within a single day [8,9].

This is different from AMH - it's a marker of a specific monthly process rather than an overall reserve indicator, and it reflects what happened in one cycle rather than a general trend. Confirming ovulation is one input among several for anyone trying to understand their cycle, alongside cycle tracking and, where relevant, a conversation with a GP or fertility specialist.

Broader hormone panels: FSH, LH and oestradiol

A broader reproductive hormone panel - covering FSH, oestradiol, LH and progesterone - gives a wider snapshot of reproductive hormone status than any single marker alone. The timing of the blood draw matters a great deal here: FSH and oestradiol are only meaningful when measured in the early part of the cycle (typically days 2-4), while progesterone is read in the luteal phase [8]. Interpreted together, and alongside AMH, these markers describe cycle and reserve status - but, like AMH, they don't predict whether or when conception will occur [10,11].

The Fertility Test covers this broader hormone panel - FSH, oestradiol, LH and progesterone. (A separate Pregnancy Confirmation Blood Test measures hCG to confirm an established pregnancy - a different question entirely.)

Semen analysis: what it measures for male fertility

For men, semen analysis measures sperm count/concentration, motility (movement) and morphology (shape) against standard WHO reference values - the core markers used to assess male fertility factors. Male factors contribute significantly to many couples' difficulty conceiving, which is why semen analysis is typically one of the first tests considered alongside female-factor testing - not instead of it [12].

As with the markers above, a semen analysis result reflects specific sperm parameters at one point in time. It does not on its own predict whether or when conception will occur, and results are best discussed with a GP or fertility specialist - particularly where results fall outside typical ranges. Because sperm parameters naturally vary between samples, an abnormal result is typically repeated (usually at least a month later) before it's considered diagnostic [12].

Vaginal microbiome: a different kind of marker

The vaginal microbiome - the balance of bacteria naturally present - is increasingly discussed in relation to reproductive health, since a balanced microbiome is relevant to comfort, infection risk and the broader vaginal environment. This is a different kind of marker to the hormone tests above: it reflects microbial balance rather than hormone levels, and disruptions are linked to symptoms like abnormal discharge, itching or discomfort.

A growing body of research has linked a Lactobacillus-dominant vaginal microbiome - particularly Lactobacillus crispatus - with higher rates of implantation and pregnancy after fertility treatment, while other bacterial profiles have been associated with lower odds of pregnancy. But the most recent systematic reviews conclude the evidence isn't yet strong enough to use microbiome profiling in fertility decision-making, with data on live births especially limited [14]. This remains an area of active research rather than an established fertility test - best understood as contextual information about vaginal health, not a fertility predictor.

The Vaginal Microbiome Test assesses vaginal pH and markers relevant to bacterial vaginosis and aerobic vaginitis - useful context alongside hormone-based fertility testing, not a replacement for it.

Bringing it together

No currently available fertility test - AMH, a broader hormone panel, or a semen analysis - can reliably predict whether or when a given individual or couple will conceive [3,4,12]. Each has a specific, narrower purpose: anticipating IVF stimulation response, confirming that ovulation likely occurred, or assessing semen parameters at one point in time. What they can do, together, is give a GP or fertility specialist a clearer set of markers to work from - ovarian reserve, ovulation status, sperm parameters, hormone balance and vaginal health - rather than starting a fertility conversation with no data at all.

This article is general information only and does not replace advice from a doctor or fertility specialist. None of the tests described predict the likelihood or timing of conception. Anyone experiencing difficulty conceiving, or with specific fertility concerns, should speak with a GP or fertility specialist directly.

Image of Lindi Collett (B.Dietetics)
Lindi Collett (B.Dietetics)

Lindi is i-screen's dietitian with a strong focus on nutrigenomics, chronic disease prevention, and personalised health strategies. With over 15 years of experience, she specialises in interpreting genetic insights to empower clients to optimise their wellness through tailored nutrition and lifestyle interventions. As part of the i-screen team, Lindi is dedicated to bridging the gap between genetic potential and practical health solutions.

References:
  1. Malhotra N, Gupta P, Kamboj S, Chaturvedi P, Kutum R. Age-specific variations in ovarian reserves in healthy fertile and infertile women: a cross-sectional study. PLoS One. 2024;19(10):e0308865. doi:10.1371/journal.pone.0308865.
  2. Committee on Gynecologic Practice. The use of antimüllerian hormone in women not seeking fertility care. Obstet Gynecol. 2019;133(4):e274-e278. doi:10.1097/AOG.0000000000003162.
  3. Lin C, Jing M, Zhu W, et al. The value of anti-Müllerian hormone in the prediction of spontaneous pregnancy: a systematic review and meta-analysis. Front Endocrinol. 2021;12:695157. doi:10.3389/fendo.2021.695157.
  4. Steiner AZ, Pritchard D, Stanczyk FZ, et al. Association between biomarkers of ovarian reserve and infertility among older women of reproductive age. JAMA. 2017;318(14):1367-1376. doi:10.1001/jama.2017.14588.
  5. Nelson SM, Davis SR, Kalantaridou S, et al. Anti-Müllerian hormone for the diagnosis and prediction of menopause: a systematic review. Hum Reprod Update. 2023;29(3):327-346. doi:10.1093/humupd/dmac045.
  6. Finkelstein JS, Lee H, Karlamangla A, et al. Antimüllerian hormone and impending menopause in late reproductive age: the Study of Women's Health Across the Nation. J Clin Endocrinol Metab. 2020;105(4):dgz283. doi:10.1210/clinem/dgz283.
  7. Practice Committee of the American Society for Reproductive Medicine. Testing and interpreting measures of ovarian reserve: a committee opinion. Fertil Steril. 2020;114(6):1151-1157. doi:10.1016/j.fertnstert.2020.09.134.
  8. Fertility evaluation of infertile women: a committee opinion. Fertil Steril. 2021;116(5):1255-1265. doi:10.1016/j.fertnstert.2021.08.038.
  9. Practice Committees of the American Society for Reproductive Medicine and the Society for Reproductive Endocrinology and Infertility. Diagnosis and treatment of luteal phase deficiency: a committee opinion. Fertil Steril. 2021;115(6):1416-1423. doi:10.1016/j.fertnstert.2021.02.010.
  10. Salemi F, Jambarsang S, Kheirkhah A, et al. The best ovarian reserve marker to predict ovarian response following controlled ovarian hyperstimulation: a systematic review and meta-analysis. Syst Rev. 2024;13(1):303. doi:10.1186/s13643-024-02684-0.
  11. Maman E, Adashi EY, Baum M, Hourvitz A. Prediction of ovulation: new insight into an old challenge. Sci Rep. 2023;13(1):20003. doi:10.1038/s41598-023-47241-2.
  12. Carson SA, Kallen AN. Diagnosis and management of infertility: a review. JAMA. 2021;326(1):65-76. doi:10.1001/jama.2021.4788.
  13. de Kat AC, van der Schouw YT, Eijkemans MJ, et al. Back to the basics of ovarian aging: a population-based study on longitudinal anti-Müllerian hormone decline. BMC Med. 2016;14(1):151. doi:10.1186/s12916-016-0699-y.
  14. Shabana H, Gudnadottir U, Hugerth LW, Rodriguez-Wallberg KA, Brusselaers N. Vaginal microbiome composition and medically assisted reproduction outcomes: a systematic review and network meta-analysis of 2289 women across three WHO regions. Hum Reprod Update. 2026. doi:10.1093/humupd/dmag033.

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