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Test Kit

Methylation Pathways Test

65 tests included
This test is designed as a follow up to the Genetic Methylation Test. The Methylation Pathways Test is a urine test that analyses key metabolic markers to reveal how your body manages nutrients, energy, and detoxification. Paired with the Genetic Methylation Test, this panel provides a comprehensive view of your current metabolic health.
$375 AUD
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Genetic methylation testing provides valuable insights into your body’s potential by identifying variations in key genes that influence methylation. But genes are only part of the picture — they reveal your blueprint, but not necessarily how your body is functioning in real time.

The Methylation Pathways Check measures compounds in your urine which reflect the effectiveness of critical processes in your body. By analysing these metabolites or methylation pathways, you'll gain insights into your body's:

  • Cellular energy production
  • Detoxification efficiency
  • Neurotransmitter balance
  • Oxidative stress and antioxidant capacity
  • Vitamin and nutrient status
  • Gut health (bacterial or yeast overgrowth)

What's included

This Organic Acids Test looks at more than 130 chemical by-products in your urine. Each one comes from a different part of how your body makes energy, handles nutrients, processes brain chemicals, manages inflammation, and interacts with the microbes in your gut and the chemicals in your environment. By looking at the overall pattern, we get a detailed snapshot of how these systems are running.

Gut & Microbial BalanceCellular EnergyNeurotransmitter ActivityOxidative Stress & DetoxificationNutrient & Vitamin StatusToxin Exposure

This area looks at chemicals produced by the trillions of microbes living in your gut, giving a rough read on which microbial groups are more or less active than usual. It won't tell you exactly which species are present, but it can flag patterns worth a closer look with your clinician.

Benzoic AcidHippuric Acid4-Hydroxybenzoic Acid (4-HBA)3,4-Dihydroxybenzoic Acid (3,4-DHBA)3-Hydroxyphenylacetic Acid (3-HPAA)Furancarbonylglycine3,4-Dihydroxyphenylpropionic Acid (DHPPA)Phenylacetic Acid (PAA)Phenylpropionic Acid3-Phenyllactic Acid (3-PLA)2-Hydroxyphenylacetic Acid (2-HPAA)4-Hydroxyphenyllactic Acid (4-HPLA)3-Hydroxypropionic Acid (3-HPA)4-Cresol4-Hydroxyphenylacetic Acid (4-HPAA)Indoleacetic Acid (IAA)3-(3-Hydroxyphenyl)-3-hydroxypropionic Acid (HPHPA)ArabinitolArabinoseCitramalic Acid3-Oxoglutaric Acid5-Hydroxymethyl-2-furoic AcidFuran-2,5-dicarboxylic AcidTartaric AcidTricarballylic AcidOxalic AcidGlycolic AcidGlyceric Acid

Your cells turn food into energy through a series of chemical steps inside the mitochondria, and this area measures the by-products of those steps to see how smoothly the process is running. Isolated findings here are common and often shift with diet or recent activity - the pattern across markers matters more than any single result.

Glucose (OA)Pyruvic AcidLactic AcidCitric Acidcis-Aconitic AcidIsocitric Acid2-Oxoglutaric AcidSuccinic AcidFumaric AcidMalic AcidAcetoacetic Acid3-Hydroxybutyric Acid2-Hydroxybutyric Acid (alpha-HBA)Methylsuccinic Acid2-Methylglutaric Acid3-Methylglutaconic Acid2-Hydroxyglutaric Acid3-Hydroxyglutaric AcidMalonic AcidMevalonolactone2,4-Dihydroxybutanoic AcidN-Acetylaspartic AcidAdipic AcidPimelic AcidSuberic AcidAzelaic AcidSebacic AcidEthylmalonic AcidPropionylglycineN-ButyrylglycineIsovalerylglycineN-(2-Methylbutyryl)glycine3-MethylcrotonylglycineTiglylglycineHexanoylglycineSuberylglycine

After your body uses brain chemicals like dopamine, serotonin, and adrenaline, it breaks them down into smaller by-products that pass out in urine, which this area measures as an indirect read on messenger activity. These markers are influenced by diet, sleep, stress, caffeine and medications, so a single reading is rarely diagnostic on its own. This area also calculates key ratios between these markers, which can reveal whether inflammation is diverting resources away from brain chemical production.

3,4-Dihydroxyphenylacetic Acid (DOPAC)Homovanillic Acid (HVA)HVA/DOPAC Ratio3-Methyl-4-hydroxyphenylglycol (MHPG)Vanillylmandelic Acid (VMA)HVA/VMA Ratio5-Hydroxyindoleacetic Acid (5-HIAA)Kynurenic AcidQuinolinic AcidPicolinic AcidKynurenic/Quinolinic RatioQuinolinic/5-HIAA RatioCortisol

This area looks at how well your body is handling everyday cellular wear-and-tear and clearing the substances your liver needs to process - medications, hormones, alcohol, and the by-products of your own metabolism. Temporary elevations are common after intense exercise, illness, or high alcohol intake, and are usually not concerning in isolation. It also includes a direct inflammation marker and an antioxidant status marker, giving a broader picture of your body’s inflammatory and protective balance.

Pyroglutamic AcidN-Acetylcysteine (NAC)8-hydroxy-deoxyguanosineQuercetinLeukotriene E4Glucaric Acid2-Hydroxyhippuric AcidOrotic Acid

This area combines two different views of your nutrient status: "functional" markers that rise when your body is working harder to use a vitamin, and direct measurements of vitamins passing through in urine. Together they give a rough indication of which nutrients may be in higher demand, though a blood test is still the gold standard for confirming any specific deficiency.

Methylmalonic Acid (MMA)Formiminoglutamic Acid (FIGLU)Xanthurenic Acid3-Hydroxyisovaleric AcidGlutaric Acid (Vitamin B2)Pantothenic Acid (Vitamin B5)Pyridoxic Acid (Vitamin B6)Ascorbic Acid (Vitamin C)Methylcitric Acid (Biotin/Vitamin H)3-Hydroxy-3-methylglutaric Acid (CoQ10)2-Oxoisovaleric Acid2-Oxoisocaproic Acid3-Methyl-2-oxovaleric Acid3-Methylglutaric Acid2-Hydroxyisovaleric Acid2-Hydroxyisocaproic AcidSuccinylacetonePhenylpyruvic AcidHomogentisic AcidN-Acetylphenylalanine2-Oxobutyric Acid2-Oxoadipic AcidMandelic AcidN-Acetylglycine2-Oxo-4-methiolbutyric Acid (KMBA)Guanidinoacetic Acid (GAA)Guanidinobutyric AcidUracilThymine

This area screens for chemical traces that your body leaves behind after processing common environmental substances - solvents found in paints, fuels, and adhesives; plasticisers in food packaging and plastic products; preservatives in cosmetics and personal care products; and compounds from vehicle exhaust and cigarette smoke. A raised reading doesn’t necessarily mean you’re being harmed - it’s a prompt to think about where the exposure might be coming from and whether it can be reduced.

t,t-Muconic Acid2-Methylhippuric Acid3-Methylhippuric Acid4-Methylhippuric AcidPhenylglyoxylic AcidMandelic Acid + Phenylglyoxylic AcidPhthalic AcidMonoethyl Phthalate2-Hydroxyisobutyric Acid3,4-Dimethylhippuric Acid4-Hydroxyhippuric Acid5-MethylcytosineCreatinine - Urine Spot

Test instructions

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You’ll receive your urine test kit in the mail, along with logistics for your sample collection.

calendar-instructions instruction-kit

Results for this test typically available in 2-3 weeks and will be published in your online dashboard.

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Common Questions